Isolation of antibacterial material-producing bacteria from saline soil
Authors
Abstract:
Saline soils are widely spread in Iran. These intact soils are a great source for the isolation of new bacteria with highly functional metabolites in biotechnology. Actinomycete strains were isolated on starch casein agar and ISP2 with different concentrations of sodium chloride (0, 5 and 10%) from treated soil samples. Pure colonies were cultured on a casein glycerol medium. After complete growth, the plates were covered with a thin layer of Muller Hinton Agar (1%) containing methicillin-resistant Staphylococcus aureus (MRSA). Active metabolites of selected strains were extracted and their antibacterial activities analyzed by agar well diffusion method. 38% of isolates produced antibiotics against the pathogen. The metabolites produced by act-2 and act-5 isolates, which had a more effective inhibition zone against MRSA, were extracted and anti MRSA activity of act-5 extract was shown. The antimicrobial activity of act-5 against other bacteria was also investigated and the bacterium was identified. In this study halophilic actinomycetes producing bioactive compounds were isolated from the saline soils of Qom and the anti-MRSA potential of their metabolites was investigated for the first time. The results of this study show the potential of saline soil actinomycetes for the production of useful metabolites.
similar resources
Isolation of antifungal bacteria from soil samples
Six strains of Bacillus cereus exhibiting antifungal activity were isolated from local environment and optimized for growth conditions. The isolates appeared neutrophiles, grew best at aeration and were represented by thermophiles and mesophiles. Growth inhibition zones of varying diameters were observed when the isolates were tested against Saccharomyces cerevisiae on nutrient agar. Largest in...
full textIsolation of the Bacteria Producing Antibiotic from the Phylosphere of two Families of Fabaceae and Asteraceae
Abstract Background and Objective: Antibiotics are the chemical compounds, which are produced by microorganisms, belong to a larger group of antimicrobial compounds that are used for treatment of infections caused by microorganisms. Material and Methods: the isolates of four plant species, ...
full textIsolation and Identification of a Rare Actinomycete with Antibacterial Activity from Saline Region of Iran
Background: The appearance of multi-drug resistant microorganisms is becoming a global problem. Already several strategies have been employed to overcome antibiotic resistance issue. Developing new antimicrobial compounds from microbial sources could be a beneficial solution. Hence screening programs in order to discover new antibiotics from microbial entities are interesting. Because of high c...
full textIsolation and Optimization of Ethanol Producing Bacteria from Natural Environments of Mazandaran Province in Iran
Ethanol producing bacteria are useful in industrial production of biofuel. There are interesting for screening of active bacteria from natural resources and introduce to biofuel industries. The present study aims to isolation of ethanol producing bacteria with characterization, optimization, and evaluation of their ethanol productivity. Samples from various fruits, plant saps and soils were scr...
full textIsolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island
ABSTRACT Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-...
full textMy Resources
Journal title
volume 5 issue 4
pages 372- 378
publication date 2019-03
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023